Determination of Antimicrobial Activities in Isolated Soil Fungi from Kyonemangay, Whakhema Township, Ayewarwady Region of Myanmar
Keywords:
Soil microorganism, Antimicrobial activities, Paper disc diffusion assay, Myanmar, Kyonemangay, Whakhema TownshipAbstract
The soil microorganisms were isolated from five different places of soil samples collected from Kyonemangay, Whakhema Township. For the isolating of fungi, soil samples were collected from 30cm depth after removing the surface layer of soil. Some physicochemical properties of soil samples were determined such as pH range, moisture content, soil temperature and soil texture. Soil fungi were isolated by soil dilution methods and chemical treatment methods. Ten soil fungi were isolated, and their morphological characters were studied. The isolated soil fungi were used to test antimicrobial activities by the paper disc diffusion assay methods against eight test organisms such as Agrobacterium tumefaciens, Bacillus Subtilis, Bacillus pumilus, Candida albicans, Staphylococcus aureus, Escherichia coli, Salmonella typhi and Micrococcus luteus. According to the results of antimicrobial activities, TTSM-01, TTSM-03 and TTSM-07 showed antimicrobial activities against bacillus subtilis and candida albicans. TTSM-03 was selected for further investigations because it exhibited the highest antifungal activity against Candida albicans with 25.9 mm clear zone.
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